Title : Gas chromatography-mass spectrometry with high-performance liquid chromatography prepurification for monitoring the endonuclease III-mediated excision of 5-hydroxy-5,6-dihydrothymine and 5,6-dihydrothymine from gamma-irradiated DNA.

Pub. Date : 1998 Jun 12

PMID : 9686872






1 Functional Relationships(s)
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1 Examples of application of the assay are provided with the measurement of the E. coli endonuclease III-mediated excision of 5-hydroxy-5,6-dihydrothymine and 5,6-dihydrothymine from samples of gamma-irradiated DNA in the presence of cysteine. Cysteine endonuclease III Escherichia coli