Title : Interaction of hydrophobic probes with the apoenzyme of pig heart lipoamide dehydrogenase.

Pub. Date : 1976 May

PMID : 956145






3 Functional Relationships(s)
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1 The interaction of hydrophobic probes, 8-anilinonaphthalene-1-sulfonate (ANS) and 4-benzoylamido-4"-aminostilbene-2, 2"-disulfonate (MBAS), with pig heart lipoamide dehydrogenase [NADH: lipoamide oxidoreductase, EC 1.6.4.3] was investigated. 8-anilino-1-naphthalenesulfonic acid dihydrolipoamide dehydrogenase Sus scrofa
2 When ANS or MBAS was mixed with the apoenzyme of lipoamide dehydrogenase, the fluorescence quantum yield, of each dye was enhancedd markedly and the emission maxima concurrently shifted to the blue. 8-anilino-1-naphthalenesulfonic acid dihydrolipoamide dehydrogenase Sus scrofa
3 These results indicate that ANS or MBAS bound to the apoenzyme of lipoamide dehydrogenase is situated in a hydrophobic region of the apoenzyme molecule. 8-anilino-1-naphthalenesulfonic acid dihydrolipoamide dehydrogenase Sus scrofa