Title : The yeast CWH41 gene encodes glucosidase I.

Pub. Date : 1997 Oct

PMID : 9363442






2 Functional Relationships(s)
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1 Following 1 h labeling of cells with [3H]mannose, analysis by high pressure liquid chromatography of the labeled N-linked oligosaccharides, combined with treatment with jack bean alpha mannosidase and yeast glucosidase I, shows that the oligosaccharides isolated form a cwh41 delta null mutant are fully glucosylated, retaining the three terminal glucose residues, whereas the oligosaccharides from CWH41 cells do not have any glucose residues. n-linked oligosaccharides mannosyl-oligosaccharide glucosidase Saccharomyces cerevisiae S288C
2 Following 1 h labeling of cells with [3H]mannose, analysis by high pressure liquid chromatography of the labeled N-linked oligosaccharides, combined with treatment with jack bean alpha mannosidase and yeast glucosidase I, shows that the oligosaccharides isolated form a cwh41 delta null mutant are fully glucosylated, retaining the three terminal glucose residues, whereas the oligosaccharides from CWH41 cells do not have any glucose residues. n-linked oligosaccharides mannosyl-oligosaccharide glucosidase Saccharomyces cerevisiae S288C