Title : Escherichia coli flavodoxin sepharose as an affinity resin for cytochromes P450 and use to identify a putative cytochrome P450c17/3beta-hydroxysteroid dehydrogenase interaction.

Pub. Date : 1997 Nov 1

PMID : 9344469






2 Functional Relationships(s)
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1 Intriguingly, a major protein copurifying with P450c17 and P450c21 was identified as 3beta-hydroxysteroid dehydrogenase (3beta-HSD) which was subsequently found not to directly bind Fld Sepharose. Sepharose 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase Bos taurus
2 Purified bovine 3beta-HSD covalently linked to Sepharose can bind recombinant bovine P450c17, an interaction which is partially disrupted upon mild heat denaturation of P450c17 or by the nonionic detergent Emulgen. Sepharose 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase Bos taurus