Title : The equilibrium intermediate of beta-lactoglobulin with non-native alpha-helical structure.

Pub. Date : 1997 Jun 20

PMID : 9217253






2 Functional Relationships(s)
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1 To understand the mechanism that stabilizes the non-native intermediate, we characterized by circular dichroism (CD) the equilibrium unfolding transition of beta-lactoglobulin induced by guanidine hydrochloride (Gdn-HCl) at pH 2 and 4 degrees C. The unfolding transition measured by near-UV CD preceded the transition measured by far-UV CD, indicating the accumulation of the intermediate state. Guanidine beta-lactoglobulin Bos taurus
2 To understand the mechanism that stabilizes the non-native intermediate, we characterized by circular dichroism (CD) the equilibrium unfolding transition of beta-lactoglobulin induced by guanidine hydrochloride (Gdn-HCl) at pH 2 and 4 degrees C. The unfolding transition measured by near-UV CD preceded the transition measured by far-UV CD, indicating the accumulation of the intermediate state. Guanidine beta-lactoglobulin Bos taurus