Pub. Date : 1997 Mar
PMID : 9058597
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Involvement of activator protein-1 and nuclear factor-kappaB transcription factors in the control of the DT-diaphorase expression induced by mitomycin C treatment. | Mitomycin | NAD(P)H quinone dehydrogenase 1 | Homo sapiens |
| 2 | The antitumor antibiotic mitomycin C is activated by several bioreductive enzymes, including DT-diaphorase. | Mitomycin | NAD(P)H quinone dehydrogenase 1 | Homo sapiens |
| 3 | In HT29 cells, mitomycin C treatment results in the induction of DT-diaphorase as reflected in elevated steady state DT-diaphorase mRNA levels. | Mitomycin | NAD(P)H quinone dehydrogenase 1 | Homo sapiens |
| 4 | In HT29 cells, mitomycin C treatment results in the induction of DT-diaphorase as reflected in elevated steady state DT-diaphorase mRNA levels. | Mitomycin | NAD(P)H quinone dehydrogenase 1 | Homo sapiens |
| 5 | To investigate the molecular basis of the change in transcriptional activity caused by mitomycin C treatment, electrophoretic mobility shift assays were used to demonstrate the induction of nuclear factor binding to elements in the 5" flanking region of the DT-diaphorase gene. | Mitomycin | NAD(P)H quinone dehydrogenase 1 | Homo sapiens |
| 6 | Treatment with mitomycin C resulted in a 5.5-fold increase in the expression of a chloramphenicol acetyltransferase construct containing 3 kb of DT-diaphorase promoter sequence. | Mitomycin | NAD(P)H quinone dehydrogenase 1 | Homo sapiens |
| 7 | Using a series of deletion mutations based on this full-length construct, we found that two regions of the DT-diaphorase promoter region, positions -346 to -588 (containing the AP-1 element) and positions -785 to -890 (containing the NF-kappaB element) are required for the full expression of the mitomycin C response. | Mitomycin | NAD(P)H quinone dehydrogenase 1 | Homo sapiens |
| 8 | These data suggest that mitomycin C induces the overexpression of DT-diaphorase through a mechanism involving both the AP-1 and NF-kappaB response elements and that inducibility depends on a novel factor binding element. | Mitomycin | NAD(P)H quinone dehydrogenase 1 | Homo sapiens |