Title : Soluble expression in Escherichia coli, one-step purification, and characterization of Chinese hamster dihydrofolate reductase.

Pub. Date : 1997 Mar

PMID : 9056490






1 Functional Relationships(s)
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1 The steady-state kinetic parameters, dissociation constants for binary complexes of dihydrofolate, NADPH, and methotrexate with ch-DHFR, and the inhibitor constant of methotrexate have also been determined. dihydrofolate dihydrofolate reductase Escherichia coli