Title : Evidence for negative cooperativity among human thyrotropin receptors overexpressed in mammalian cells.

Pub. Date : 1996 Nov

PMID : 8895321






3 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 Similarly, [125I]TSH cross-linking to the surface of intact CHO cells revealed a progressive increase in TSH-binding sites with dihydrofolate reductase minigene amplification, with a 12.8-fold increase in TSHR in TSHR-10,000 vs. TSHR-0 cells. Thyrotropin thyrotropin receptor Cricetulus griseus
2 Similarly, [125I]TSH cross-linking to the surface of intact CHO cells revealed a progressive increase in TSH-binding sites with dihydrofolate reductase minigene amplification, with a 12.8-fold increase in TSHR in TSHR-10,000 vs. TSHR-0 cells. Thyrotropin thyrotropin receptor Cricetulus griseus
3 We assessed the kinetics of TSH binding to CHO cells overexpressing the TSHR using [125I]TSH in the presence of increasing concentrations of unlabeled TSH as well as by attempted saturation with labeled ligand. Thyrotropin thyrotropin receptor Cricetulus griseus