Title : Development and validation of a spectrophotometric assay for measuring the activity of NADH: cytochrome b5 reductase in human tumour cells.

Pub. Date : 1996 Oct

PMID : 8883403






4 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 Using p-hydroxymercuribenzoate (pHMB) as an inhibitor of B5R, we have quantified the contribution of B5R to the NADH-dependent reduction of cytochrome c and to the overall reduction of cytochrome c in the DT-diaphorase assay. NAD cytochrome c, somatic Homo sapiens
2 Using p-hydroxymercuribenzoate (pHMB) as an inhibitor of B5R, we have quantified the contribution of B5R to the NADH-dependent reduction of cytochrome c and to the overall reduction of cytochrome c in the DT-diaphorase assay. NAD cytochrome c, somatic Homo sapiens
3 Thus, simply measuring the NADH-dependent reduction of cytochrome c consistently overestimated B5R activity. NAD cytochrome c, somatic Homo sapiens
4 Therefore, we have developed a spectrophotometric assay for measuring B5R activity as the pHMB-inhibitable NADH-dependent reduction of cytochrome c. NAD cytochrome c, somatic Homo sapiens