Title : Transcriptional control of the heme oxygenase gene in mouse M1 cells during their TPA-induced differentiation into macrophages.

Pub. Date : 1996 Sep 1

PMID : 8872603






4 Functional Relationships(s)
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1 Upon treatment with TPA, H2O2 was produced first, the nuclear proto-oncogenes fos and jun were activated, and then the HO gene was activated. Tetradecanoylphorbol Acetate FBJ osteosarcoma oncogene Mus musculus
2 The extent of transcriptional activation of the fos, jun, and HO genes in M1 cells treated with TPA was reduced by a specific inhibitor of C-kinase and a scavenger of oxygen radicals. Tetradecanoylphorbol Acetate FBJ osteosarcoma oncogene Mus musculus
3 When M1 cells were treated with H2O2, essentially the same level of transcription of the HO gene was observed, but the extent of transcriptional activation of the fos and jun genes was about half of the treatment with TPA. Tetradecanoylphorbol Acetate FBJ osteosarcoma oncogene Mus musculus
4 Super-shift assays using the TRE of the HO gene revealed that the Fos and Jun proteins from nuclei of M1 cells treated with TPA bound to the TRE, and same assays using DNA with the NF-kappa B motif also revealed that the active NF-kappa B protein from M1 cells treated with H2O2 or TPA also bound to the corresponding motif. Tetradecanoylphorbol Acetate FBJ osteosarcoma oncogene Mus musculus