Title : A Zn(II)-binding site engineered into retinol-binding protein exhibits metal-ion specificity and allows highly efficient affinity purification with a newly designed metal ligand.

Pub. Date : 1996 Aug

PMID : 8807898






1 Functional Relationships(s)
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1 BACKGROUND: The Zn(II)-binding site from the active center of human carbonic anhydrase II, formed by three His side chains, can be grafted onto the recombinant serum retinol-binding protein (RBP). Histidine carbonic anhydrase 2 Homo sapiens