Pub. Date : 1996 Jul 19
PMID : 8663268
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The interaction of Saccharomyces cerevisiae Ras2p with the catalytic domain of the GDP/GTP exchange factors (GEFs) mouse CDC25(Mm), yeast Cdc25p, and Sdc25p was analyzed by introducing the substitution R80D/N81D into Ras2p S24N, a mutant that is shown to interfere with the Ras2p wild type (wt)-GEF interaction by forming a stable complex. | Guanosine Triphosphate | Ras family GTPase RAS2 | Saccharomyces cerevisiae S288C |
| 2 | The interaction of Saccharomyces cerevisiae Ras2p with the catalytic domain of the GDP/GTP exchange factors (GEFs) mouse CDC25(Mm), yeast Cdc25p, and Sdc25p was analyzed by introducing the substitution R80D/N81D into Ras2p S24N, a mutant that is shown to interfere with the Ras2p wild type (wt)-GEF interaction by forming a stable complex. | Guanosine Triphosphate | Ras family GTPase RAS2 | Saccharomyces cerevisiae S288C |
| 3 | The interaction of Saccharomyces cerevisiae Ras2p with the catalytic domain of the GDP/GTP exchange factors (GEFs) mouse CDC25(Mm), yeast Cdc25p, and Sdc25p was analyzed by introducing the substitution R80D/N81D into Ras2p S24N, a mutant that is shown to interfere with the Ras2p wild type (wt)-GEF interaction by forming a stable complex. | Guanosine Triphosphate | Ras family GTPase RAS2 | Saccharomyces cerevisiae S288C |
| 4 | The affinity of Ras2p S24N/R80D/N81D for GDP and GTP was decreased 3 and 4 orders of magnitude, respectively, like that of Ras2p S24N, whereas the double mutant behaved as Ras2p wt. | Guanosine Triphosphate | Ras family GTPase RAS2 | Saccharomyces cerevisiae S288C |
| 5 | Like Ras2p S24N and unlike Ras2p R80D/N81D, the GTP-bound triple mutant did not activate adenylyl cyclase. | Guanosine Triphosphate | Ras family GTPase RAS2 | Saccharomyces cerevisiae S288C |
| 6 | Thus, the triple mutant and Ras2p S24N have opposite properties toward the binding to GEF but similarly modified behaviors toward GDP, GTP, and adenylyl cyclase. | Guanosine Triphosphate | Ras family GTPase RAS2 | Saccharomyces cerevisiae S288C |