Pub. Date : 1996 Feb
PMID : 8655628
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin, 12-O-tetradecanoylphorbol-13-acetate and 17 beta-estradiol on estrogen receptor regulation in MCF-7 human breast cancer cells. | Tetradecanoylphorbol Acetate | estrogen receptor 1 | Homo sapiens |
| 2 | Comparative studies were conducted with E2 and 12-O-tetradecanoylphorbol-13-acetate (TPA), as both compounds are known to suppress ER expression. | Tetradecanoylphorbol Acetate | estrogen receptor 1 | Homo sapiens |
| 3 | Comparative studies were conducted with E2 and 12-O-tetradecanoylphorbol-13-acetate (TPA), as both compounds are known to suppress ER expression. | Tetradecanoylphorbol Acetate | estrogen receptor 1 | Homo sapiens |
| 4 | Our results indicate that 1 nM E2 and 100 nM TPA both suppress ER mRNA levels as early as 4 h after exposure and to 33.6% and 16.5% of control levels, respectively, after 72 h. In contrast, no significant effect on ER mRNA levels was attributed to exposure to 10 nM TCDD. | Tetradecanoylphorbol Acetate | estrogen receptor 1 | Homo sapiens |
| 5 | Our results indicate that 1 nM E2 and 100 nM TPA both suppress ER mRNA levels as early as 4 h after exposure and to 33.6% and 16.5% of control levels, respectively, after 72 h. In contrast, no significant effect on ER mRNA levels was attributed to exposure to 10 nM TCDD. | Tetradecanoylphorbol Acetate | estrogen receptor 1 | Homo sapiens |
| 6 | A greater than 50% reduction in positive staining was observed by ER-ICA after 72 h exposure to 1 nM E2 and to 100 nM TPA, while only an 11% reduction in positive staining was observed with 10 nM TCDD. | Tetradecanoylphorbol Acetate | estrogen receptor 1 | Homo sapiens |
| 7 | In conclusion, while TPA and E2 effectively down-regulate ER expression, TCDD, under antiestrogenic conditions, has little if any effect on total ER levels in MCF-7 cells, and thus ER modulation is probably not necessary for the suppression of estrogenic activity in MCF-7 cells by TCDD. | Tetradecanoylphorbol Acetate | estrogen receptor 1 | Homo sapiens |