Title : Mechanism of ubiquitin conjugating enzyme E2-230K: catalysis involving a thiol relay?

Pub. Date : 1996 Feb 6

PMID : 8634298






1 Functional Relationships(s)
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1 We show that phenylarsenoxides potently inhibit E2-230K, apparently by binding to vicinal Cys residues of the enzyme: bound aminophenylarsenoxide partially protects the enzyme against inactivation by N-ethylmalemide (NEM), and prior enzyme inactivation with NEM blocks enzyme binding to immobilized phenylarsenoxide. Cysteine ubiquitin conjugating enzyme E2 O Homo sapiens