Pub. Date : 1996 Mar
PMID : 8603613
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Competitive quantitative RT-PCR demonstrated that the level of TGF-beta1 messenger RNA increased approximately 7-fold when cells were treated with 10(-7) M DHT. | Dihydrotestosterone | transforming growth factor beta 1 | Homo sapiens |
| 2 | Results of Western blot analysis showed a dramatic increase in the level of latent TGF-beta1 protein in cell lysates with increasing concentrations of DHT. | Dihydrotestosterone | transforming growth factor beta 1 | Homo sapiens |
| 3 | In addition, results of enzyme-linked immunoadsorbent assay for TGF-beta1 indicated that treatment of LNCaP cells with DHT led to a dose-dependent increase in both total and biologically active TGF-beta1 in the conditioned media. | Dihydrotestosterone | transforming growth factor beta 1 | Homo sapiens |
| 4 | In addition, results of enzyme-linked immunoadsorbent assay for TGF-beta1 indicated that treatment of LNCaP cells with DHT led to a dose-dependent increase in both total and biologically active TGF-beta1 in the conditioned media. | Dihydrotestosterone | transforming growth factor beta 1 | Homo sapiens |
| 5 | First, TGF-beta1 neutralizing antibody was added to the culture medium with varying concentrations of DHT. | Dihydrotestosterone | transforming growth factor beta 1 | Homo sapiens |
| 6 | These observations, taken together, indicate that TGF-beta1 mediates at least in part the growth arrest observed at the high concentration of DHT in LNCaP cells. | Dihydrotestosterone | transforming growth factor beta 1 | Homo sapiens |