Pub. Date : 1993 Aug
PMID : 8361957
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Selective extraction of alkaline phosphatase and 5"-nucleotidase from milk fat globule membranes by a single phase n-butanol procedure. | 1-Butanol | 5'-nucleotidase | Bos taurus |
| 2 | For 5"-nucleotidase, higher n-butanol concentrations lead to loss of activity, while lower concentrations were ineffective in extracting the enzyme. | 1-Butanol | 5'-nucleotidase | Bos taurus |
| 3 | When extractions were performed at 0 degrees C, similar yields were obtained for alkaline phosphatase extraction with 8% (v/v) n-butanol, but 5"-nucleotidase extraction required 10% (v/v) n-butanol for similar yields. | 1-Butanol | 5'-nucleotidase | Bos taurus |
| 4 | However, 5"-nucleotidase was less susceptible to denaturation during extraction at 0 degrees C. The Km values and substrate specificities for both alkaline phosphatase and 5"-nucleotidase were unchanged by extraction with 8% (v/v) n-butanol. | 1-Butanol | 5'-nucleotidase | Bos taurus |