Pub. Date : 1994 Jan 7
PMID : 8276782
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Differential structural requirements of heparin and heparan sulfate proteoglycans that promote binding of basic fibroblast growth factor to its receptor. | Heparin | fibroblast growth factor 2 | Bos taurus |
| 2 | The capacity of various species of heparin and heparan sulfate (HS) to promote bFGF receptor binding was investigated using both Chinese hamster ovary mutant cells deficient in cell surface HSPG and a soluble bFGF receptor-alkaline phosphatase fusion protein. | Heparin | fibroblast growth factor 2 | Bos taurus |
| 3 | O-Sulfation in heparin was found to be critical for its capacity to promote binding of bFGF to its receptors. | Heparin | fibroblast growth factor 2 | Bos taurus |
| 4 | The highest level of bFGF-receptor binding was achieved in the presence of over-sulfated heparin fragments (% sulfur > 14) regardless of whether the N-position was sulfated or acetylated. | Heparin | fibroblast growth factor 2 | Bos taurus |
| 5 | Unlike receptor binding of bFGF which requires oligosaccharides containing at least 8-10 sugar units, displacement of heparin- or HS-bound bFGF was obtained by oligosaccharides containing as little as four sugar units and by an N-sulfated, O-desulfated heparin fragment (% sulfur = 5.3). | Heparin | fibroblast growth factor 2 | Bos taurus |
| 6 | Unlike receptor binding of bFGF which requires oligosaccharides containing at least 8-10 sugar units, displacement of heparin- or HS-bound bFGF was obtained by oligosaccharides containing as little as four sugar units and by an N-sulfated, O-desulfated heparin fragment (% sulfur = 5.3). | Heparin | fibroblast growth factor 2 | Bos taurus |
| 7 | Moreover, most of these species of HS inhibited in a dose-dependent manner the restoration of bFGF-receptor binding induced by heparin or by total HSPG. | Heparin | fibroblast growth factor 2 | Bos taurus |
| 8 | These results suggest the involvement of defined heparin-like oligosaccharide sequences and unique species of cell surface and extracellular matrix HS in the regulation of bFGF receptor binding and biological activity. | Heparin | fibroblast growth factor 2 | Bos taurus |