Title : Kinetics of interaction between normal and proline 12 Ras and the GTPase-activating proteins, p120-GAP and neurofibromin. The significance of the intrinsic GTPase rate in determining the transforming ability of ras.

Pub. Date : 1993 Dec 25

PMID : 8262937






2 Functional Relationships(s)
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1 Single turnover and equilibrium binding measurements on the interaction of Gly-12 and Pro-12 Ras.GTP with the catalytic domains of the GTPase-activating proteins, p120-GAP and neurofibromin, have been made utilizing fluorescent 2"(3")O-(N-methylanthraniloyl)-nucleotides. Guanosine Triphosphate RAS p21 protein activator 1 Homo sapiens
2 Both p120-GAP and neurofibromin increased the rate constant of the GTP hydrolysis step of Pro-12 Ras, but the maximal activation at 30 degrees C was 120-fold and 560-fold, as compared with 70,000- and 52,000-fold, with Gly-12 Ras. Guanosine Triphosphate RAS p21 protein activator 1 Homo sapiens