Title : The H(+)-ATPase from reticulocyte endosomes reconstituted into liposomes acts as an iron transporter.

Pub. Date : 1994 Apr 8

PMID : 8144605






4 Functional Relationships(s)
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1 The H(+)-ATPase from reticulocyte endosomes was purified and reconstituted into liposomes, and protein-dependent iron transport was observed. Iron dynein axonemal heavy chain 8 Homo sapiens
2 Upon addition of ascorbate, an initial efflux of 2.9 +/- 0.3 x 10(-2) mumol of iron/mg of ATPase/min and 56 +/-7% of total internal Fe(II) was detected by formation of the Fe(II)-FerroZine complex with an absorbance at 562 nm or radioactivity of 59Fe(II)-FerroZine following separation using gel filtration. Iron dynein axonemal heavy chain 8 Homo sapiens
3 The amount of iron transported was decreased 51 or 39% by 100 microM N,N"-dicyclohexylcarbodiimide or 70 microM of the ATPase inhibitor 7-chloro-4-nitrobenz-2-oxa-1,3-diazole. Iron dynein axonemal heavy chain 8 Homo sapiens
4 These results suggest that this vacuolar H(+)-ATPase may transport iron. Iron dynein axonemal heavy chain 8 Homo sapiens