Title : Regeneration of active receptor recognition domains on the B subunit of cholera toxin by formation of hybrids from chemically inactivated derivatives.

Pub. Date : 1994 Sep 8

PMID : 8086501






1 Functional Relationships(s)
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1 The number of active binding sites on hybrid CTB was determined from: (i) titration with the oligosaccharide moiety of GM1 (oligo-GM1) and monitoring the reversal of the Trp fluorescence quenching by iodide ions and (ii) rapid gel filtration over a superdex HR column of a mixture of hybrid CTB and an excess of 3H-labeled oligo-GM1. Iodides phosphate cytidylyltransferase 1B, choline Homo sapiens