Title : Purification of histaminase (diamine oxidase) from human pregnancy plasma by affinity chromatography.

Pub. Date : 1975 Aug 26

PMID : 808238






2 Functional Relationships(s)
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1 The procedure is based upon the binding of histaminase to cadaverine, a diamine substrate for the enzyme, which is coupled to Sepharose. Cadaverine amine oxidase copper containing 1 Homo sapiens
2 The purification technique has the following characteristics: (1) in optimal experiments, 3000-fold purification of enzyme was obtained; (2) the yield of enzyme was as great as 25%; (3) the binding of histaminase to the amine groups of the cadaverine appears to represent a true "affinity" phenomenon since enzyme bound to DEAE-cellulose under neutral pH conditions was eluted at much lower concentrations of NaCl (less than 0.4 M). Cadaverine amine oxidase copper containing 1 Homo sapiens