Title : Functional role of the cis-acting elements in human monocyte chemotactic protein-1 gene in the regulation of its expression by phorbol ester in human glioblastoma cells.

Pub. Date : 1994 Dec 21

PMID : 7891669






4 Functional Relationships(s)
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Protein Name
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1 The expression of MCP-1 gene can be induced by lipopolysaccharides (LPS), phorbol esters (TPA) and several cytokines. Tetradecanoylphorbol Acetate C-C motif chemokine ligand 2 Homo sapiens
2 We tested whether the two putative TPA-responsive elements (TREs) and one kappa B enhancer-like region found in the MCP-1 promoter region, are involved in this regulation of MCP-1 gene expression. Tetradecanoylphorbol Acetate C-C motif chemokine ligand 2 Homo sapiens
3 The 5" untranslated region of MCP-1 gene was linked to chloramphenicol acetyl transferase (CAT) reporter gene and transfected into human glioblastoma cells in which endogenous MCP gene expression was found to be stimulated by TPA and tumor necrosis factor-alpha (TNF-alpha). Tetradecanoylphorbol Acetate C-C motif chemokine ligand 2 Homo sapiens
4 The 128 bp 5"-flanking region containing one TRE was adequate for basal promoter activity but the presence of both TREs in the MCP-1 promoter region were needed to give TPA responsive enhancement (2.5 fold) of expression of the marker gene. Tetradecanoylphorbol Acetate C-C motif chemokine ligand 2 Homo sapiens