Title : High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2.

Pub. Date : 1994 Dec

PMID : 7811063






2 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Ura- auxotrophs were obtained by selecting for resistance to 5-fluoroorotic acid and were identified as ura3 mutants by transformation with P. stipitis URA3. Uracil orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C
2 Ura- auxotrophs were obtained by selecting for resistance to 5-fluoroorotic acid and were identified as ura3 mutants by transformation with P. stipitis URA3. Uracil orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C