Title : Structure-assisted redesign of a protein-zinc-binding site with femtomolar affinity.

Pub. Date : 1995 May 23

PMID : 7761440






1 Functional Relationships(s)
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1 In contrast, the three-dimensional structure of threonine-199-->histidine (T199H) CAII, determined to 2.25-Angstrum resolution, indicates that the engineered imidazole side chain rotates away from the metal and does not coordinate to zinc; this results in a weaker zinc-binding site. Histidine carbonic anhydrase 2 Homo sapiens