Title : Spatial and electrogenic properties of superoxide-producing cytochrome b-559 incorporated into liposomes.

Pub. Date : 1995 May 10

PMID : 7748884






3 Functional Relationships(s)
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1 Purified cytochrome b-559 reconstituted into liposomes, consisting of certain azolectin-based phospholipid mixtures, is capable of NADPH-supported FAD-dependent superoxide (O2-) production in the absence of cytosolic activators. NADP mitochondrially encoded cytochrome b Homo sapiens
2 In the present report, representing an extension of our earlier investigations, two types of vesicle-incorporated and reflavinated cytochrome b-559 preparation were distinguished by their ability to catalyze vectorial electrogenic or scalar electron transport from NADPH to oxygen. NADP mitochondrially encoded cytochrome b Homo sapiens
3 This can be explained by the existence of two distinct membranal locations of cytochrome b-559, with NADPH-binding and O2-reducing sites exposed on different or on the same side of the membrane. NADP mitochondrially encoded cytochrome b Homo sapiens