Title : Protein denaturation by addition and removal of acetonitrile: application to tryptic digestion of acetylcholinesterase.

Pub. Date : 1995 Jan 1

PMID : 7710103






1 Functional Relationships(s)
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1 Bovine erythrocyte acetylcholinesterase was prepared for tryptic digestion by radiomethylating with [14C]HCHO and NaCNBH3, cleaving with purified bacterial phosphatidylinositol-specific phospholipase C to remove the lipid portion of the glycoinositol phospholipid anchor, and reducing and alkylating the intersubunit disulfide bonds. Carbon-14 acetylcholinesterase Bos taurus