Title : Isolation of interleukin 2-induced immediate-early genes.

Pub. Date : 1993 Apr 1

PMID : 7681987






2 Functional Relationships(s)
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1 To increase the frequency of IL-2-induced transcripts represented in the library, the protein synthesis inhibitor cycloheximide was included during the 2-hr IL-2 stimulation to superinduce gene expression, and the uridine analogue 4-thiouridine was utilized to enable selective purification of newly synthesized transcripts. Cycloheximide interleukin 2 Homo sapiens
2 To increase the frequency of IL-2-induced transcripts represented in the library, the protein synthesis inhibitor cycloheximide was included during the 2-hr IL-2 stimulation to superinduce gene expression, and the uridine analogue 4-thiouridine was utilized to enable selective purification of newly synthesized transcripts. Cycloheximide interleukin 2 Homo sapiens