Pub. Date : 1993 Mar
PMID : 7679999
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The addition of phorbol 12-myristate 13-acetate (PMA) to Hep 3B cells subsequently grown under hypoxic conditions resulted in a dose-dependent inhibition of hypoxia-induced Epo production by as much as 95 +/- 1% with half-maximal inhibition at 8 ng/mL. | Tetradecanoylphorbol Acetate | erythropoietin | Homo sapiens |
| 2 | The addition of phorbol 12-myristate 13-acetate (PMA) to Hep 3B cells subsequently grown under hypoxic conditions resulted in a dose-dependent inhibition of hypoxia-induced Epo production by as much as 95 +/- 1% with half-maximal inhibition at 8 ng/mL. | Tetradecanoylphorbol Acetate | erythropoietin | Homo sapiens |
| 3 | By Northern blot analysis, Epo mRNA levels were correspondingly decreased after treatment with PMA. | Tetradecanoylphorbol Acetate | erythropoietin | Homo sapiens |
| 4 | The PMA-induced inhibition of hypoxia-induced Epo production was shown to occur as early as 3 hours after PMA addition, suggesting that the initial activation, rather than the subsequent decrease in protein kinase C activity, is of primary importance. | Tetradecanoylphorbol Acetate | erythropoietin | Homo sapiens |
| 5 | The PMA-induced inhibition of hypoxia-induced Epo production was shown to occur as early as 3 hours after PMA addition, suggesting that the initial activation, rather than the subsequent decrease in protein kinase C activity, is of primary importance. | Tetradecanoylphorbol Acetate | erythropoietin | Homo sapiens |
| 6 | The relative specificity of the PMA-induced inhibition of Epo production was demonstrated by 1) the finding that overall protein and RNA synthesis were not similarly decreased as measured by 3H-leucine and 3H-uridine pulse labeling studies and 2) the observation that the biologically inactive phorbol ester, 4 alpha-phorbol didecanoate, failed to have any effect on hypoxia-induced Epo production. | Tetradecanoylphorbol Acetate | erythropoietin | Homo sapiens |
| 7 | The relative specificity of the PMA-induced inhibition of Epo production was demonstrated by 1) the finding that overall protein and RNA synthesis were not similarly decreased as measured by 3H-leucine and 3H-uridine pulse labeling studies and 2) the observation that the biologically inactive phorbol ester, 4 alpha-phorbol didecanoate, failed to have any effect on hypoxia-induced Epo production. | Tetradecanoylphorbol Acetate | erythropoietin | Homo sapiens |