Title : Conformational changes of bovine bone osteonectin induced by interaction with calcium.

Pub. Date : 1995 Jun

PMID : 7648487






3 Functional Relationships(s)
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1 By calcium titration using fluorescence spectrometry, it was demonstrated that FITC-osteonectin labeled in the presence of 2 mM CaCl2 showed a much higher affinity for calcium ions than did that labeled in the absence of calcium ions. Calcium Chloride secreted protein acidic and cysteine rich Bos taurus
2 Furthermore, it was found that the efficacy of labeling in this specific binding site was three times higher in the FITC-osteonectin labeled in the presence of 2 mM CaCl2 than in that labeled in the absence of calcium. Calcium Chloride secreted protein acidic and cysteine rich Bos taurus
3 The results indicate that in the presence of 2 mM CaCl2 the microenvironment around Lys174 of osteonectin was more open to modification than in the absence of calcium. Calcium Chloride secreted protein acidic and cysteine rich Bos taurus