Title : Effects of 12-O-tetradecanoylphorbol-13-acetate on estrogen receptor activity in MCF-7 cells.

Pub. Date : 1995 Oct 20

PMID : 7559663






11 Functional Relationships(s)
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1 Effects of 12-O-tetradecanoylphorbol-13-acetate on estrogen receptor activity in MCF-7 cells. Tetradecanoylphorbol Acetate estrogen receptor 1 Homo sapiens
2 The effects of long term treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) on estrogen receptor (ER) expression in the human breast cancer cell line, MCF-7, were studied. Tetradecanoylphorbol Acetate estrogen receptor 1 Homo sapiens
3 The effects of long term treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) on estrogen receptor (ER) expression in the human breast cancer cell line, MCF-7, were studied. Tetradecanoylphorbol Acetate estrogen receptor 1 Homo sapiens
4 The effects of long term treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) on estrogen receptor (ER) expression in the human breast cancer cell line, MCF-7, were studied. Tetradecanoylphorbol Acetate estrogen receptor 1 Homo sapiens
5 The effects of long term treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) on estrogen receptor (ER) expression in the human breast cancer cell line, MCF-7, were studied. Tetradecanoylphorbol Acetate estrogen receptor 1 Homo sapiens
6 Treatment of cells with 100 nM TPA resulted in an 80% decrease in the level of ER protein and a parallel decrease in ER mRNA and binding capacity. Tetradecanoylphorbol Acetate estrogen receptor 1 Homo sapiens
7 Treatment of cells with 100 nM TPA resulted in an 80% decrease in the level of ER protein and a parallel decrease in ER mRNA and binding capacity. Tetradecanoylphorbol Acetate estrogen receptor 1 Homo sapiens
8 Following removal of TPA from the medium, the level of ER protein and mRNA returned to control values; however, the receptor failed to bind estradiol. Tetradecanoylphorbol Acetate estrogen receptor 1 Homo sapiens
9 In addition, TPA treatment blocked transcription from an estrogen response element in transient transfection assays and inhibited ER binding to its response element in a DNA mobility shift assay. Tetradecanoylphorbol Acetate estrogen receptor 1 Homo sapiens
10 Mixing experiments suggest that TPA induces/activates a factor which interacts with the ER to block binding of estradiol. Tetradecanoylphorbol Acetate estrogen receptor 1 Homo sapiens
11 The effects of TPA on ER levels and binding capacity were concentration-dependent. Tetradecanoylphorbol Acetate estrogen receptor 1 Homo sapiens