Title : Characterization of mammalian C-CDC25Mm exchange factor and kinetic properties of the exchange reaction intermediate p21.C-CDC25Mm.

Pub. Date : 1995 Sep 26

PMID : 7547978






4 Functional Relationships(s)
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1 Formation of a complex with nucleotide-free H-ras p21 could be analyzed on native gel electrophoresis by combining C-CDC25Mm and p21.GDP, as the result of the fast separation of GDP from p21. Guanosine Diphosphate H3 histone pseudogene 16 Homo sapiens
2 Formation of a complex with nucleotide-free H-ras p21 could be analyzed on native gel electrophoresis by combining C-CDC25Mm and p21.GDP, as the result of the fast separation of GDP from p21. Guanosine Diphosphate H3 histone pseudogene 16 Homo sapiens
3 Therefore, in order to obtain highly purified heterodimer in preparative amounts, p21.GDP and C-CDC25Mm were exposed to an electric field and the complex purified by anionic chromatography. Guanosine Diphosphate H3 histone pseudogene 16 Homo sapiens
4 The "on-rate" of the nucleotide on the p21.C-CDC25Mm complex was similar for GDP and GTP and was little increased vs that on p21 alone. Guanosine Diphosphate H3 histone pseudogene 16 Homo sapiens