Title : Kinetics of inorganic phosphate release during the interaction of p21ras with the GTPase-activating proteins, p120-GAP and neurofibromin.

Pub. Date : 1995 Nov 28

PMID : 7492562






2 Functional Relationships(s)
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1 The rate of GTP hydrolysis on p21ras is accelerated by approximately 10(5) times by the catalytic domains of GTPase-activating proteins (GAPs), p120-GAP (GAP-344) or neurofibromin (NF1-334). Guanosine Triphosphate HRas proto-oncogene, GTPase Homo sapiens
2 Measurements were made in real time with a stopped-flow apparatus, in which the p21ras complex with the 2",3"-methanthraniloyl analogue of GTP (mantGTP) was mixed with the GAP in the presence of this Pi probe. Guanosine Triphosphate HRas proto-oncogene, GTPase Homo sapiens