Title : Extraction of the apo B cluster from human low density lipoprotein with Tween 80.

Pub. Date : 1980 Nov

PMID : 7462184






6 Functional Relationships(s)
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1 Extraction of the apo B cluster from human low density lipoprotein with Tween 80. Polysorbates apolipoprotein B Homo sapiens
2 The properties of Tween 80-extracted apoprotein (apo B) were studied by sedimentation velocity and sedimentation equilibrium measurements, gel chromatography, electron microscopy and chemical cross-linking. Polysorbates apolipoprotein B Homo sapiens
3 A further increase of Tween 80 concentration caused the all-or-none dissociation of the entire mass of apo B from LDL. Polysorbates apolipoprotein B Homo sapiens
4 The remaining lipid core of LDL was not destroyed by detergent at 25 degrees C, but it was at 35 degrees C. The Tween 80-extracted apo B was purified by gel chromatography and its molecular weight and partial specific volume were determined by sedimentation equilibrium analysis in H2O and D2O buffers. Polysorbates apolipoprotein B Homo sapiens
5 This result was confirmed by chemical cross-linking of apo B in Tween 80. Polysorbates apolipoprotein B Homo sapiens
6 Under an electron microscope the apo B-Tween 80 complex appeared as a flexible string, a little less than 1,000 A in length and 50 to 60 A in width. Polysorbates apolipoprotein B Homo sapiens