Pub. Date : 1983 Oct 1
PMID : 6639055
12 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The comparative kinetics of soluble and heparin-Sepharose-immobilized bovine lipoprotein lipase. | Heparin | lipoprotein lipase | Bos taurus |
| 2 | Hence kinetic parameters of S-LPL and heparin-Sepharose-immobilized LPL (B-LPL) were compared. | Heparin | lipoprotein lipase | Bos taurus |
| 3 | Dixon plots of experiments with S-LPL indicated that heparin is a competitive inhibitor against both C-II and TG, and that the binding of either C-II or heparin to the enzyme is a mutually exclusive event. | Heparin | lipoprotein lipase | Bos taurus |
| 4 | From the Dixon plots, the dissociation constant Ki for the LPL:heparin binary complex was determined to be 5.0 X 10(-8) M. In contrast to the heparin inhibitory effect on LPL activity against triolein, heparin had no effect on the esterase activity of LPL against p-nitrophenylacetate or p-nitrophenylbutyrate. | Heparin | lipoprotein lipase | Bos taurus |
| 5 | From the Dixon plots, the dissociation constant Ki for the LPL:heparin binary complex was determined to be 5.0 X 10(-8) M. In contrast to the heparin inhibitory effect on LPL activity against triolein, heparin had no effect on the esterase activity of LPL against p-nitrophenylacetate or p-nitrophenylbutyrate. | Heparin | lipoprotein lipase | Bos taurus |
| 6 | From the Dixon plots, the dissociation constant Ki for the LPL:heparin binary complex was determined to be 5.0 X 10(-8) M. In contrast to the heparin inhibitory effect on LPL activity against triolein, heparin had no effect on the esterase activity of LPL against p-nitrophenylacetate or p-nitrophenylbutyrate. | Heparin | lipoprotein lipase | Bos taurus |
| 7 | From the Dixon plots, the dissociation constant Ki for the LPL:heparin binary complex was determined to be 5.0 X 10(-8) M. In contrast to the heparin inhibitory effect on LPL activity against triolein, heparin had no effect on the esterase activity of LPL against p-nitrophenylacetate or p-nitrophenylbutyrate. | Heparin | lipoprotein lipase | Bos taurus |
| 8 | From the Dixon plots, the dissociation constant Ki for the LPL:heparin binary complex was determined to be 5.0 X 10(-8) M. In contrast to the heparin inhibitory effect on LPL activity against triolein, heparin had no effect on the esterase activity of LPL against p-nitrophenylacetate or p-nitrophenylbutyrate. | Heparin | lipoprotein lipase | Bos taurus |
| 9 | It is concluded that most of the LPL bound to heparin-Sepharose is probably inaccessible to substrate, hence a low Vmax. | Heparin | lipoprotein lipase | Bos taurus |
| 10 | However, Km (C-II) and Km (TG) were higher for B-LPL due to the competitive inhibitory effect of heparin on LPL. | Heparin | lipoprotein lipase | Bos taurus |
| 11 | However, Km (C-II) and Km (TG) were higher for B-LPL due to the competitive inhibitory effect of heparin on LPL. | Heparin | lipoprotein lipase | Bos taurus |
| 12 | Consistent with these kinetic analyses and with the use of human very low density lipoproteins (VLDL) as substrate, S-LPL, even in the presence of heparin, was found to have an apparent rate of lipolysis of VLDL approximately ninefold greater than B-LPL. | Heparin | lipoprotein lipase | Bos taurus |