Title : Residues Cys-1 and Cys-79 are not essential for refolding of reduced-denatured kringle 4 fragment of human plasminogen.

Pub. Date : 1984 Jun 28

PMID : 6329306






2 Functional Relationships(s)
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1 It was shown previously that the Cys-1-Cys-79 disulphide bond forms in the last step of refolding of kringle 4 and that this bond is not essential for the lysine-Sepharose affinity of the kringle 4 fragment (Trexler, M. and Patthy, L. (1983) Proc. disulphide cystin 1 Homo sapiens
2 Here we show that kringle 4, carboxymethylated on Cys-1 and Cys-79, regains its lysine-Sepharose affinity following denaturation and reductive cleavage of its disulphide bonds. disulphide cystin 1 Homo sapiens