Title : A specific ultrastructural stain for arylsulfatase A activity in human cultured fibroblasts.

Pub. Date : 1984 Jun

PMID : 6202736






1 Functional Relationships(s)
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1 A staining reaction was developed to specifically detect arylsulfatase A activity in the presence of arylsulfatases B and C. Nitrocatechol, generated by all arylsulfatases from the substrate p-nitrocatechol sulfate, can be coupled to produce Hatchett "s brown which reacts with 3,3"-diaminobenzidine to yield an osmiophilic polymer visible under the electron microscope. nitrocatechol arylsulfatase A Homo sapiens