Title : An assay for estradiol preceptors using a staphylococcal protein-A--estradiol antibody adsorbent.

Pub. Date : 1979 Dec 15

PMID : 397197






1 Functional Relationships(s)
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1 Since the affinity constant for estradiol of antiestradiol antibodies is about 10(8) M-1 while those for breast estrogen-receptor complexes are about 10(9) to 10(10) M-1, it is possible to remove the free steroid from a reaction mixture containing tissue cytosol, radio-labelled estradiol, and the antiestradiol bacterial adsorbent by pelleting the bacteria at low-speed centrifugation. Steroids estrogen receptor 1 Homo sapiens