Title : Irreversible inhibition of pancreatic lipase by bis-p-nitrophenyl methylphosphonate.

Pub. Date : 1985 May 20

PMID : 3922786






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1 The reaction of porcine pancreatic lipase with an organophosphorus compound bis-p-nitrophenyl methylphosphonate (BNMP) resulted in the complete and irreversible inhibition of lipase activity on tributyrin emulsion (25 degrees C, pH 7.5, 40 mM Na-veronal-HCl buffer) whereas the activity of the enzyme on p-nitrophenyl acetate solution remained unchanged. 4-nitrophenyl acetate pancreatic lipase Homo sapiens