Title : Simultaneous production of two types of beta-lactamase in Escherichia coli and Providencia stuartii.

Pub. Date : 1978 Oct

PMID : 365308






1 Functional Relationships(s)
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1 Determination and identification of beta-lactamase activity were achieved by combining several up-to-date methods including (i) neutralization by anti-beta-lactamase sera, (ii) purification by affinity chromatography on cephalosporin C linked Sepharose 4B, (iii) determination of substrate specificity and kinetic values (Km and Vmax) by a computerized microacidimetric method, and (iv) isoelectric focusing. cephalosporin C beta-lactamase Escherichia coli