Title : Expression of rat intestinal fatty acid-binding protein in Escherichia coli. Purification and comparison of ligand binding characteristics with that of Escherichia coli-derived rat liver fatty acid-binding protein.

Pub. Date : 1987 Apr 25

PMID : 3553183






5 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 It is homologous to rat liver fatty acid-binding protein (L-FABP), a 14,273-Da cytosolic protein which is found in enterocytes as well as hepatocytes. Fatty Acids fatty acid binding protein 1 Rattus norvegicus
2 All of the fatty acids associated with I-FABP were saturated while 30% of the E. coli fatty acids bound to L-FABP were unsaturated (16:1, 18:1, 18:2). Fatty Acids fatty acid binding protein 1 Rattus norvegicus
3 We directly analyzed the ability of I- and L-FABP to bind fatty acids of different chain length and degree of saturation using a hydroxyalkoxypropyl dextran-based assay. Fatty Acids fatty acid binding protein 1 Rattus norvegicus
4 Scatchard analysis revealed that each mole of L-FABP can bind up to 2 mol of long chain fatty acid while each mole of I-FABP can bind only 1 mole of fatty acid. Fatty Acids fatty acid binding protein 1 Rattus norvegicus
5 L-FABP exhibited a relatively higher affinity for unsaturated fatty acids (oleate, arachidonate) than for saturated fatty acid (palmitate). Fatty Acids fatty acid binding protein 1 Rattus norvegicus