Title : The SARS-CoV-2 Spike Glycoprotein Directly Binds Exogeneous Sialic Acids: A NMR View.

Pub. Date : 2022 Apr 25

PMID : 35191576






4 Functional Relationships(s)
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1 The interaction of the SARS CoV2 spike glycoprotein with two sialic acid-containing trisaccharides (a2,3 and a2,6 sialyl N-Acetyllactosamine) has been demonstrated by NMR. N-Acetylneuraminic Acid surface glycoprotein Severe acute respiratory syndrome coronavirus 2
2 The NMR-based distinction between the signals of those sialic acids in the glycans covalently attached to the spike protein and those belonging to the exogenous a2,3 and a2,6 sialyl N-Acetyllactosamine ligands has been achieved by synthesizing uniformly 13C-labelled trisaccharides at the sialic acid and galactose moieties. N-Acetylneuraminic Acid surface glycoprotein Severe acute respiratory syndrome coronavirus 2
3 Additional experiments with the spike protein in the presence of a specific antibody for the N-terminal domain and with the isolated receptor binding and N-terminal domains of the spike protein unambiguously show that the sialic acid binding site is located at the N-terminal domain. N-Acetylneuraminic Acid surface glycoprotein Severe acute respiratory syndrome coronavirus 2
4 Additional experiments with the spike protein in the presence of a specific antibody for the N-terminal domain and with the isolated receptor binding and N-terminal domains of the spike protein unambiguously show that the sialic acid binding site is located at the N-terminal domain. N-Acetylneuraminic Acid surface glycoprotein Severe acute respiratory syndrome coronavirus 2