Pub. Date : 2022 Jan
PMID : 34536557
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Hinokitiol-induced decreases of tyrosinase and microphthalmia-associated transcription factor are mediated by the endoplasmic reticulum-associated degradation pathway in human melanoma cells. | beta-thujaplicin | melanocyte inducing transcription factor | Homo sapiens |
| 2 | As a result, hinokitiol treatment decreased TYR protein level in a time- and dose-dependent manner in G361 human melanoma cells, while MITF protein level was decreased only at higher concentrations after 3 days treatment. | beta-thujaplicin | melanocyte inducing transcription factor | Homo sapiens |
| 3 | Notably, the mRNA levels of TYR and MITF were slightly increased by hinokitiol treatment. | beta-thujaplicin | melanocyte inducing transcription factor | Homo sapiens |
| 4 | Importantly, co-treatment of ERAD inhibitor with hinokitiol restored the protein levels of TYR and MITF to approximately 30% and 20% of total those in untreated control cells, respectively. | beta-thujaplicin | melanocyte inducing transcription factor | Homo sapiens |
| 5 | Hinokitiol affected the ER homeostasis as well as degradation of TYR and MITF in two human melanoma cell lines, G361 and HT-144, but the changes of ER-stress markers under the hinokitiol treatment were different in the two human melanoma cell lines. | beta-thujaplicin | melanocyte inducing transcription factor | Homo sapiens |
| 6 | Taken together, these observations indicate that hinokitiol may induce ER stress and trigger the degradation of unfolded newly synthesizing TYR and MITF via the ERAD pathway. | beta-thujaplicin | melanocyte inducing transcription factor | Homo sapiens |