Title : Inducibility, but not stability, of atrial fibrillation is increased by NOX2 overexpression in mice.

Pub. Date : 2021 Sep 28

PMID : 33483749






7 Functional Relationships(s)
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1 AIMS: Gp91-containing NADPH oxidases (NOX2) are a significant source of myocardial superoxide production. Superoxides cytochrome b-245, beta polypeptide Mus musculus
2 METHODS AND RESULTS: NOX2-Tg mice showed a 2-2.5-fold higher atrial protein content of NOX2 compared with wild-type (WT) controls, which was associated with a significant (2-fold) increase in NADPH-stimulated superoxide production (2-hydroxyethidium by HPLC) in left and right atrial tissue homogenates (P = 0.004 and P = 0.019, respectively). Superoxides cytochrome b-245, beta polypeptide Mus musculus
3 METHODS AND RESULTS: NOX2-Tg mice showed a 2-2.5-fold higher atrial protein content of NOX2 compared with wild-type (WT) controls, which was associated with a significant (2-fold) increase in NADPH-stimulated superoxide production (2-hydroxyethidium by HPLC) in left and right atrial tissue homogenates (P = 0.004 and P = 0.019, respectively). Superoxides cytochrome b-245, beta polypeptide Mus musculus
4 Finally, treatment with atorvastatin significantly inhibited atrial superoxide production in NOX2-Tg but had no effect on AF induction in either genotype. Superoxides cytochrome b-245, beta polypeptide Mus musculus
5 CONCLUSIONS: Together, these data indicate that whilst atrial NOX2 overexpression may contribute to atrial arrhythmogenesis, NOX2-derived superoxide production does not affect the electrical and structural properties of the atrial myocardium. Superoxides cytochrome b-245, beta polypeptide Mus musculus
6 Short-term treatment with atorvastatin normalized atrial superoxide in NOX2-Tg mice without affecting AF susceptibility. Superoxides cytochrome b-245, beta polypeptide Mus musculus
7 Together these findings indicate that atrial NOX2-derived superoxide is more likely a biomarker of AF risk than a primary driver of AF development, and that NOX2 inhibition is unlikely to prevent the new-onset of AF. Superoxides cytochrome b-245, beta polypeptide Mus musculus