Title : Directed evolution of the transcription factor Gal4 for development of an improved transcriptional regulation system in Saccharomyces cerevisiae.

Pub. Date : 2020 Dec

PMID : 33220863






3 Functional Relationships(s)
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1 During the preliminary screening, five positive Gal4 variants were isolated based on the lycopene-indicated high-throughput screening method. Lycopene galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C
2 Through combinatorial site-directed mutagenesis, the best variant Gal4T406A/V413A was obtained, which successfully increased the transcription of PGAL-driven lycopene pathway genes and led to 48 % higher lycopene accumulation relative to the wild-type Gal4. Lycopene galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C
3 Through combinatorial site-directed mutagenesis, the best variant Gal4T406A/V413A was obtained, which successfully increased the transcription of PGAL-driven lycopene pathway genes and led to 48 % higher lycopene accumulation relative to the wild-type Gal4. Lycopene galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C