Title : Phoneutria toxin PnTx3-5 inhibits TRPV1 channel with antinociceptive action in an orofacial pain model.

Pub. Date : 2020 Jan 1

PMID : 31647972






3 Functional Relationships(s)
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1 Capsaicin, an agonist of TRPV1, evokes intracellular [Ca2+] transients and glutamate release from perfused trigeminal ganglion. Calcium transient receptor potential cation channel subfamily V member 1 Homo sapiens
2 PnTx3-5 (40 nM) and SB-366791 (3 muM) also inhibited the capsaicin-induced increase in intracellular Ca2+ in HEK293 cells transfected with TRPV1 by 75 +- 16 % and 84 +- 3.2 %, respectively. Calcium transient receptor potential cation channel subfamily V member 1 Homo sapiens
3 In HEK293 cells transfected with TRPA1, cinnamaldehyde (30 muM) generated an increase in intracellular Ca2+ that was blocked by the TRPA1 antagonist HC-030031 (10 muM, 89% inhibition), but not by PnTx3-5 (40 nM), indicating selectivity of the toxin for TRPV1. Calcium transient receptor potential cation channel subfamily V member 1 Homo sapiens