Title : Conversion of xanthine dehydrogenase to oxidase in ischemic rat intestine: a reevaluation.

Pub. Date : 1988 May

PMID : 3163236






1 Functional Relationships(s)
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1 Grinding intestine under liquid nitrogen and placing the powder in phosphate buffer (pH 7.0) containing thiol reductants and protease inhibitors adequately preserved total XDH + XO activity and the percentage in the oxidase form (%XO) for 24 h. Total activity in nonischemic intestine ranged from 374 nmol.min-1.g-1 in duodenum to 138 nmol.min-1.g-1 in ileum, while XO activity was approximately 19% of total activity throughout the entire small intestine. Sulfhydryl Compounds xanthine dehydrogenase Rattus norvegicus