Title : Differential modification of specificity in carbonic anhydrase catalysis.

Pub. Date : 1988 May 5

PMID : 3129421






3 Functional Relationships(s)
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1 Incubation of carbonic anhydrase II with acrolein results in a rapid, time-dependent loss of all but approximately 3-6% of the original catalytic activity toward CO2 hydration and HCO3- dehydration, with the inactivation rate being first-order in both acrolein and the enzyme. Acrolein carbonic anhydrase 2 Homo sapiens
2 Incubation of carbonic anhydrase II with acrolein results in a rapid, time-dependent loss of all but approximately 3-6% of the original catalytic activity toward CO2 hydration and HCO3- dehydration, with the inactivation rate being first-order in both acrolein and the enzyme. Acrolein carbonic anhydrase 2 Homo sapiens
3 The amount of residual CO2 hydratase activity is proportional to the molar excess of acrolein over carbonic anhydrase II with 5 histidyl and 3 lysyl residues being subject to alkylation under conditions where [acrolein] to [carbonic anhydrase II] ratio is greater than 100. Acrolein carbonic anhydrase 2 Homo sapiens