Title : GAL4 of Saccharomyces cerevisiae activates the lactose-galactose regulon of Kluyveromyces lactis and creates a new phenotype: glucose repression of the regulon.

Pub. Date : 1987 Feb

PMID : 3102945






4 Functional Relationships(s)
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1 GAL4 of Saccharomyces cerevisiae activates the lactose-galactose regulon of Kluyveromyces lactis and creates a new phenotype: glucose repression of the regulon. Lactose galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C
2 When this strain was transformed with the GAL4 positive regulatory gene of Saccharomyces cerevisiae it was able to grow on lactose or galactose as the sole carbon source. Lactose galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C
3 Transformants bearing GAL4 exhibited a 4.5-h generation time on galactose or lactose, versus 24 h for the nontransformed lac9 strain. Lactose galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C
4 In addition to restoring lactose and galactose gene expression, GAL4 in K. lactis lac9 mutant cells conferred a new phenotype, severe glucose repression of lactose and galactose-inducible enzymes. Lactose galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C