Title : The proliferative lifespan of adult sheep fibroblasts can be conditionally extended by tetracycline-inducible expression of human telomerase reverse transcriptase.

Pub. Date : 2019 Feb

PMID : 30603914






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1 The proliferative lifespan of adult sheep fibroblasts can be conditionally extended by tetracycline-inducible expression of human telomerase reverse transcriptase. Tetracycline telomerase reverse transcriptase Homo sapiens
2 Here, we report conditional extension of proliferative lifespan of adult sheep somatic cells by introducing tetracycline-inducible (Tet-on) expression of hTERT. Tetracycline telomerase reverse transcriptase Homo sapiens
3 After transfecting adult sheep fibroblasts with the vector for Tet-on induced conditional expression of hTERT, we obtained several hTERT-positive clones that exhibited extended-lifespan under the induction of tetracycline analogue, doxycycline. Tetracycline telomerase reverse transcriptase Homo sapiens