Title : Repeatability, precision, and accuracy of the enthalpies and Gibbs energies of a protein-ligand binding reaction measured by isothermal titration calorimetry.

Pub. Date : 2019 Mar

PMID : 30535510






5 Functional Relationships(s)
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1 Here, the repeatability, precision, and accuracy of the measurement of the affinity and the change in enthalpy upon acetazolamide (AZM) interaction with human carbonic anhydrase II (CA II) are discussed based on the measurements using several ITC instruments. Acetazolamide carbonic anhydrase 2 Homo sapiens
2 Here, the repeatability, precision, and accuracy of the measurement of the affinity and the change in enthalpy upon acetazolamide (AZM) interaction with human carbonic anhydrase II (CA II) are discussed based on the measurements using several ITC instruments. Acetazolamide carbonic anhydrase 2 Homo sapiens
3 Here, the repeatability, precision, and accuracy of the measurement of the affinity and the change in enthalpy upon acetazolamide (AZM) interaction with human carbonic anhydrase II (CA II) are discussed based on the measurements using several ITC instruments. Acetazolamide carbonic anhydrase 2 Homo sapiens
4 Here, the repeatability, precision, and accuracy of the measurement of the affinity and the change in enthalpy upon acetazolamide (AZM) interaction with human carbonic anhydrase II (CA II) are discussed based on the measurements using several ITC instruments. Acetazolamide carbonic anhydrase 2 Homo sapiens
5 To obtain the confidence intervals (CI) of the G and DeltaH of AZM binding to CA II, the binding reaction was repeated numerous times at the optimal concentration of 10 microM and 25 C temperature. Acetazolamide carbonic anhydrase 2 Homo sapiens